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Blue Bottle Chemistry Activity

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Blue Bottle Demonstration (continued) + ward ' s science 5100 West Henrietta Road • PO Box 92912 • Rochester, New York 14692-9012 • p: 800 962-2660 • wardsci.com Follow-up: • For best reaction, make your solutions just prior to use. • To manipulate the speed of the color change, you can vary the temperature of the solution, as well as concentration of the Methylene Blue indicator. – Speed Up Color Change a. Increase temperature of the solution. b. Increase concentration of the Glucose and Sodium Hydroxide. c. Decrease concentration of the Methylene Blue Indicator. Teaching Notes: • Methylene blue indicator is colorless when in the presence of a reducing agent but is blue in the presence of an oxidizer. • The upper layer of the solution remains in contact with the air inside the beaker and will remain slightly blue. • Photochemical Variation: – Add 2.0 g of Iron(II) Sulfate to 100mL of 0.1M Sulfuric Acid solution in a 250mL Erlenmeyer flask – Add a couple crystals of methylene blue. Mix. – The solution should turn nearly colorless when placed on an overhead projector, or under an otherwise strong light source. a. If it remains blue, dilute the solution with water. Disposal/Clean-up: • Neutralize the solution by adding dilute hydrochloric acid (0.5M, until the pH is approximately 7) • Chemical disposal should be done in accordance with all local safety and environmental regulations. Find materials for this activity at wardsci.com. Discover more free activities at wardsworld.wardsci.com MB(oxidized) + C6H1206 + NaOH —> MB(reduced) Blue Colorless MB(reduced) + C6H1206 + NaOH + O2 (via shaking) —> MB(oxidized) Colorless Blue

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