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CRISPR/Cas9 in ES Cells

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CRISPR/Cas9 in ES Cells Model Generation The JAX Model Generation core lab can generate your new mouse model by making a precise genetic modification with CRISPR/Cas9 in mouse embryonic stem (ES) cells. ∙ JAX has validated, germline-competent ES cells lines for several genetic backgrounds. ∙ Projects start with a free feasibility assessment to design your model and identify potential risks. ∙ Full-service project: JAX designs and generates all reagents and DNA, and delivers genetically validated mice directly to you. ∙ Models are generated in the US allowing for fast shipments and no quarantine. ∙ Mouse models are covered by CRISPR/Cas9 limited Use Licenses with The Broad Institute and Caribou Biosciences, Inc. Service Highlights Using the CRISPR technology in ES cells instead of zygotes increases insertion size limits and helps mitigate effects of genes that cause a severe phenotype when disrupted. ES cell modifications increase timelines, are not guaranteed to be germline transmissible, and are limited to a few common genetic backgrounds. Overview Projects are designed and conducted by our PhD-level study directors, and you will receive regular updates from your dedicated project manager. Our Process GETA TS0214 2022.09 15-25 weeks 10-12 weeks Invoice 1 Founder Pups Born Detailed Project Plan Delivered 16 weeks Invoice 2 Founder Report Delivered N1 Pups Born N1 Report Delivered Applications Genetic Backgrounds Deliverables ∙ Knock-outs (deletions and conditional) ∙ Point mutations (SNPs and conditional) ∙ Knock-ins ∙ ROSA26 Insertions ∙ C57BL/6J ∙ Other inbred strains subject to feasibility and conditions ∙ 2 heterozygous N1 mice, minimum ∙ Sperm cryopreservation included Timelines ∙ Deletions, SNP, small knock-ins: 10-11 months ∙ Knock-ins and conditional models: 11-12 months Project Plan and Design Generation and Validation of guide RNA and donor template* *Donor template required for large knock-ins only Sequence Analysis of Founder Animals ES Cell Modification Breeding of Founders to Background Strain ES Cell Modification and Screening Validation of N1 Animals

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