Product Sheets

DNA Microinjection Model Generation The Jackson Laboratory has an experienced microinjection core that can make new mouse models by microinjecting plasmid or BAC constructs, with founder mice ready to ship in as little as 13–15 weeks. GETD TS0217 2022.09 ∙ JAX routinely achieves a 90% success rate for transgenic mouse generation. ∙ Specific opportunistic and pathogen-free (SOPF) mice are provided, so no quarantine is needed when receiving mice. ∙ JAX can generate plasmids to create a random transgenic model upon request. ∙ Models are generated in the US allowing for fast shipments and no quarantine. Service Highlights Already have a plasmid or bacterial artificial chromosome (BAC) DNA construct? JAX's DNA Microinjection Service includes pronuclear microinjection of the transgenic DNA construct (plasmid or BAC) into pronuclear embryos of the genetic background of your choice. We can provide the founder mice, or breed to the N1 generation. Overview Genetic Backgrounds Deliverables Timelines ∙ Most common inbred strains ∙ All mice produced; average 3 or more ∙ Choose to receive founder or N1 mice ∙ 3-4 months to receive founder mice ∙ 7-8 months to receive N1 mice (optional) 1-3 weeks 12 weeks Invoice for Founders Founder Pups Born 16 weeks Invoice for N1 Founder Report Delivered N1 Pups Born N1 Report Delivered DNA Contructs Received DNA Preparation Sequence Analysis of Founder Animals DNA Microinjection Breeding of Founders to Background Strain Validation of N1 Animals Projects are designed and conducted by our PhD-level study directors, and you will receive regular updates from your dedicated project manager. Our Process

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