CONTACT JAX
micetech@jax.org
1.800.422.6423 (US)
1.207.288.5845 (International)
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JAX MICE AND SERVICES
JAX.ORG/JMCRS
Frequently Asked Questions
What is a random transgenic model?
Pronuclear microinjection of DNA results in an undetermined
integration, where the transgene inserts randomly into the
mouse genome. Individual founder mice may have different
integration sites, single or multiple copies, and varying
expression levels and phenotypes.
How are transgenic mice bred and maintained?
JAX recommends evaluating multiple founders for
expression and phenotype, and keeping each founder line
separate. Transgenic mice may be bred to wildtype mice of
the same genetic background or, if desired, cautiously bred
to homozygosity.
What is the purpose of breeding to the N1
generation?
By breeding to N1 and re-genotyping the mice, we can
guarantee that the genetic modification is germline
transmissible.
How large can the construct be?
We will attempt any size, however we recommend that
BACs be less than 200 kb, and that plasmids be
less than 20 kb.
How do I prepare the DNA for injection?
Please do not send already isolated DNA for your injections.
Please only send your plasmid and we will prepare the DNA
for injection at our facility to ensure that it meets our quality
standards for injection.
How do I ship the DNA to JAX?
Once your project is initiated, you will be assigned a dedicated
project manager. Your project manager will coordinate the details
of the DNA shipment, either from your facility or directly from a
commercial source.
Can you identify the transgene integration site?
Confirming transgene integration site is possible but technically
challenging in mice. Currently, we are not offering this service as
part of our standard DNA microinjection service.
How do I initiate a new project?
Your JAX sales representative will ask for additional details about
your DNA. Our study directors will then perform a feasibility
assessment, and generate a Statement of Work (SOW), which will
typically be provided to you within 1 week. If you would like to
proceed, you must sign the SOW and provide a PO to initiate the
project.
DNA Microinjection Model Generation
