Product Sheets

ES Cell Microinjection

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CONTACT JAX 1.800.422.6423 (US) (International) LEARN MORE ABOUT JAX MICE AND SERVICES JAX.ORG/JMCRS Frequently Asked Questions How do I ship the ES cells to JAX? Once your project is initiated, you will be assigned a dedicated project manager. Your project manager will coordinate the details of the ES cell shipment, either from your facility or directly from a commercial source. How many ES cell clones are microinjected? Our full-service package includes the microinjection of 2 clones with karyotyping and QA/QC, or you can pay per clone with our "lite" package. Can JAX make genetic modifications in ES cells? Yes, JAX can use CRISPR/Cas9 to make new genetic modifications in ES cell lines. How do I initiate a new project? Your JAX sales representative will ask for additional details about the ES cell clone that you would like to have injected, as well as what add on services you may need (Karyotyping, QA/ QC, breeding). A Statement of Work (SOW) will be generated and provided to you within 1 week. If you would like to proceed, you must sign the SOW and provide a PO for the full dollar amount to initiate the project. ES Cell Microinjection Model Generation How are the chimeric mice identified? The chimeric mice are identified by coat color, because we choose a host mouse that is a different coat color than the source of the ES cells. We can also genotype for the expected genetic modification. What is the purpose of breeding to the N1 generation? By breeding to N1 and re-genotyping the mice, we can guarantee that the genetic modification is germline transmissible. What does it mean if an ES cell modification is not germline transmissible? Even if the microinjection is successful and produces founder mice, the genetic modification may not be germline transmissible. This will be evidenced when you breed the founder mice to a wild type strain, and the genetic modification is lost. If this occurs with all founder mice, then a second ES cell microinjection may need to be attempted. What pathogen testing do you require for the ES cells? We test the provided ES cells for mycoplasma and murine viral contamination as part of the service offering, therefore advanced testing is not required.

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