CONTACT JAX
micetech@jax.org
1.800.422.6423 (US)
1.207.288.5845 (International)
LEARN MORE ABOUT
JAX MICE AND SERVICES
JAX.ORG/JMCRS
Frequently Asked Questions
How do I ship the ES cells to JAX?
Once your project is initiated, you will be assigned a dedicated
project manager. Your project manager will coordinate the details
of the ES cell shipment, either from your facility or directly from a
commercial source.
How many ES cell clones are microinjected?
Our full-service package includes the microinjection of 2 clones
with karyotyping and QA/QC, or you can pay per clone with our
"lite" package.
Can JAX make genetic modifications in ES cells?
Yes, JAX can use CRISPR/Cas9 to make new genetic
modifications in ES cell lines.
How do I initiate a new project?
Your JAX sales representative will ask for additional details
about the ES cell clone that you would like to have injected, as
well as what add on services you may need (Karyotyping, QA/
QC, breeding). A Statement of Work (SOW) will be generated and
provided to you within 1 week. If you would like to proceed, you
must sign the SOW and provide a PO for the full dollar amount to
initiate the project.
ES Cell Microinjection Model Generation
How are the chimeric mice identified?
The chimeric mice are identified by coat color, because we
choose a host mouse that is a different coat color than the
source of the ES cells. We can also genotype for the expected
genetic modification.
What is the purpose of breeding to the N1
generation?
By breeding to N1 and re-genotyping the mice, we can
guarantee that the genetic modification is germline
transmissible.
What does it mean if an ES cell modification is not
germline transmissible?
Even if the microinjection is successful and produces
founder mice, the genetic modification may not be germline
transmissible. This will be evidenced when you breed
the founder mice to a wild type strain, and the genetic
modification is lost. If this occurs with all founder mice, then
a second ES cell microinjection may need to be attempted.
What pathogen testing do you require for the ES
cells?
We test the provided ES cells for mycoplasma and murine
viral contamination as part of the service offering, therefore
advanced testing is not required.
